mouse anti platelet endothelial cell adhesion molecule 1  (Cell Signaling Technology Inc)

Journal: Frontiers in Physiology

Article Title: Interleukin-10 exhibit dose-dependent effects on macrophage phenotypes and cardiac remodeling after myocardial infarction

doi: 10.3389/fphys.2024.1481460

Figure Lengend Snippet: Intramyocardial injection of IL-10 enhances revascularization in the ischemic myocardium. Long-term revascularization, including angiogenesis and vasculogenesis, in the ischemic myocardium was evaluated by the presence of CD31 + endothelial cells (ECs) and αSMA + peri-vascular smooth muscle cells (SMCs), respectively, at 6 weeks post-infarction ( n = 3 per group). Perivascular SMCs defined as αSMA + cells around CD31 + ECs. Healthy (normal) hearts served as baseline controls ( n = 3). (A) Representative images of CD31 + ECs (red) and αSMA + SMCs (green) within the infarct region at the mid-infarct level from each group (scale bars: 50 μm). Nuclei were stained in blue. (B) Quantitatively, only the 250 ng group had a significantly higher density of CD31 + ECs at the infarct (p = 0.013, vs saline). (C) No significant difference in the CD31 + EC density was noted in the peri-infarct area (all p > 0.05, vs saline). (D) Only the 1,000 group had a significantly higher density of αSMA + SMCs within the infarct region (p = 0.045, vs saline). (E) No notable difference in the αSMA + SMC density in the peri-infarct area (all p > 0.05, vs saline). Note: *p < 0.05.

Article Snippet: To examine vascular endothelial cells (ECs) and smooth muscle cells (SMCs), sections were first incubated overnight at 4°C with rat anti-mouse CD31 (platelet endothelial cell adhesion molecule, PECAM-1) antibody (1:100, Becton-Dickinson Biosciences, Franklin Lakes, NJ, United States), followed by donkey anti-rat-Alexa594 IgG (1:400, Life Technologies) at RT for 1 h, and subsequently incubated with mouse anti-alpha smooth muscle actin-FITC (1:100, Sigma-Aldrich).

Techniques: Injection, Staining, Saline